Tissue regeneration

The work group 'Tissue Regeneration' of the Biomechanics and Implant Technology Research Laboratory has extensive experience in the field of cultivation and experimental procedure involving human primary bone and cartilage cells. These are isolated from femoral heads and articular cartilage and cultured in vitro.

In terms of bone regeneration, our working group exhibits detailed knowledge of two and three-dimensional cultivation of human osteoblasts in 3D scaffolds from tricalcium phosphate, tantalum and titanium. To measure the supply of oxygen to the cells and metabolic cell activity, our working group uses established static and dynamic cell culture systems with micro-sensor technology (oxygen and pH). The use
of electrical stimulation of bone cells to promote bone regeneration is also focused on.

Another area of investigation is the effect of abrasive particles (e.g. metal particles, bone cement particles, polyethylene particles) on the vitality and synthesis capacity of human bone cells, and are compared to human monocytes and macrophages, which are isolated from buffy coats.

With respect to cartilage regeneration, human chondrocytes and mesenchymal stem cells are cultivated in established 3D cell culture systems (pellet culture, alginate beads, biomaterials) under different conditions of oxygen (21% and 5%). The cells are treated with growth factors through which the differentiation potential is stimulated. In this context, we investigate the influence of
silica nanoparticles coupled with insulin-like growth factor 1 (IGF-1) on  the viability, proliferation, and chondrogenic differentiation potential of human primary articular chondrocytes and human mesenchymal stem cells.

Furthermore, biomaterials and implant surfaces are tested in vitro for various orthopaedic applications concerning their biocompatibility with various cell types (e.g. osteoblasts, chondrocytes, fibroblasts). Animal experimental analyses are also conducted in collaboration with the working group 'Experimental Biomechanics​'​.


Sponsors

Head

Staff

  • Doris Hansmann, MTA
  • Bettina Hiemer M. Sc.
  • Simone Krüger, M.Sc.
  • Sarah Zaatreh M. Sc.
  • Dipl.-Humanbiol. Josefin Ziebart
  • Students